Muhammad Rifky Yuwanto a, Mudawamah a, Sunaryo a
a Faculty of Animal Science, University of Islam Malang, Malang
Corresponding Author : mudawamah@unisma.ac.id

A B S T R A C T

This research was conducted at the Bambang Iswanto Lovebird house regency Malang and the UNISMA Halal Center Laboratory. This study aimed to measure phenotype variation and DNA of male and female Lovebird Albino with a red and black eyes. The material in this study was 40 Lovebird Albino red eyes and black eyes with male sex (MHJ) and female sex (MHB). The experimental design used descriptive quantitative. The research variables followed wing length, chest size, body length and qPCR DNA with gen TYR. The data were analyzed using a T-test unpaired. The phenotype variation in wing length, chest size, and body length between male and female Lovebird Albino red eye showed no significant effect (P>0,05). The average value on wing length was 13,7 ± 1,89 cm (MMJ), 13,2 ± 1,48 cm (MHJ), 13,7 ± 1,31 cm (MMB), 13,2 ± 2,16 cm (MHB). The average value on chest size was 6,5 ± 1,89 cm (MMJ), 6,5 ± 1,48 cm (MHJ), 6,5 ± 0,53 cm (MMB), 6,5 ± 0,53 cm (MHB). The average value for body length was 15,40 ± 0,97 cm (MMJ), 15,20 ± 0,79 cm (MHJ), 15,20 ± 0,79 cm (MMB), 15,20 ± 0,63 cm (MHB). Average values on qPCR DNA were 37,67 ± 1,79 (MMJ), 35,22 ± 3,66 (MHJ), 37,90 ± 2,12 (MMB) and 35,89 ± 2,99 (MHB). The conclusion was phenotype variation and DNA Lovebird Albino red and black eyes on males and females has no significant effect but have propensity Lovebird Albino red eyes both in male and female higher 3,79% until 6,96% compared to black eye seen from wing length and DNA.

Keywords:
Lovebird Albino, PCR, Body Morphometry

Article

Khoirur Roziqin1, Mudawamah2, Sri Susilowati2
1Program S1 Peternakan, 2Dosen Peternakan Universitas Islam Malang
Email: alarof99@gmail.com

Abstrak

Pusat Laboratorium Halal Universitas Islam Malang dan Pondok Pesantren Ash-Shiddiqy, Jln Anjasmoro No.45 G3A, Kecamatan Lawang, digunakan untuk penelitian ini. Penelitian ini membandingkan fenotipe dan DNA burung Yorkshire, Kenari Lokal, dan F1 hasil persilangan jantan untuk menentukan nilai ukuran tubuh burung Yorkshire hijau menggunakan hasil persilangan Kenari Lokal kuning pada warna bon (kuning hijau) Filial 1( F1). Dengan kenari lokal dan Yorkshire. Dengan masing-masing 10 sampel, tiga spesies burung yang digunakan dalam penelitian ini—kenari lokal kuning, burung Yorkshire hijau, dan F1 bon color (kuning hijau) — menjadi total 30 ekor. Variabel panjang badan, lingkar dada, dan DNA diamati, menggunakan DNA kuantitatif
(qPCR) dengan menggunakan gen TYR sebagai gen referensi. Metodologi yang digunakan adalah teknik deskriptif kuantitatif. Uji t tidak berpasangan digunakan untuk menilai data. Temuan mengungkapkan varian dalam fenotipe DNA dan ukuran tubuh. Tidak ada perbedaan yang signifikan secara statistik antara warna bon F1 (kuning hijau), warna kuning lokal, atau kenari Yorkshire hijau (P>0,05), meskipun ada kecenderungan perubahan yang terlihat dari nilai rata-rata. 18 0,94 cm (Yorkshire), 13,4 0,69 cm (Lokal), dan 16 0,67 cm (F1) adalah rata-rata nilai panjang badan. Pengukuran dada rata-rata untuk Yorkshire, Lokal, dan F1 masing-masing adalah 11,6 0,84 cm, 9,8 0,63 cm, dan 10,5 0,71 cm. Nilai rata-rata hasil qPCR DNA adalah 25,00 11,82 (F1), 32,75 1,14 (Lokal), dan 28,14 18,88 (Yorkshire). Hasil penelitian menunjukkan bahwa tidak ada perbedaan variasi fenotip warna bulu dan DNA antara burung Yorkshire hijau, kenari lokal kuning, dan F1. Namun ada kecenderungan ukuran tubuh burung Yorkshire hijau lebih tinggi 10,48-12,56% dibandingkan F1. Sebaliknya, nilai DNA rata-rata kenari Yorkshire hijau dan kuning lokal adalah 12,56% dan 31% lebih besar dari F1 bon (kuning hijau), dari perspektif genetik.
kata kunci : fenotipe, kenari lokal kuning, yoshire

Artikel

Mudawamah, Susilowati S. & Trijaya
Fakultas Peternakan Universitas Islam Malang

ABSTRAK

Burung kenari merupakan burung berkicau yang dipelihara bukan hanya untuk hobi tetapi sudah mengarah ke komersial dengan harga jualnya ditentukan oleh warna bulu, ukuran tubuh dan suara. Tujuan penelitian ini adalah untuk mengetahui variasi fenotipe F1 crossbreed hasil persilangan kenari lokal dengan Black throat. Metode penelitian adalah eksperimen dan data dianalisis menggunakan Chi-square. Materi penelitian yang digunakan satu ekor pejantan burung black throat, sembilan ekor indukan burung kenari lokal warna bulu hijau, starblue dan kuning. Perkawinan dilakukan dengan perkawinan alam dengan ulangan tiga kali. Variasi fenotipe yang diamati adalah warna bulu, ukuran tubuh dan suara. Hasil penelitian menunjukkan bahwa F1 crossbreed mempunyai postur tubuh lebih besar dari Black throat dan lebih kecil dari kenari lokal dengan suara lebih tinggi dan tebal dibandingkan dengan kenari lokal serta warna bulunya merupakan kombinasi kenari lokal dengan Black throat. Dari hasil análisis dengan chi-square menunjukkan bahwa variasi fenotipe persilangan black throat dengan berbagai kenari lokal tidak berbeda nyata (P > 0.05) dengan stándar penilaian Malang Canary Club. Tetapi ada kecenderungan bahwa skor hasil persilangan antara black throat dengan kenari kuning (26,6) menghasilkan rata-rata skor terendah dibandingkan dengan hijau (27) dan starblue (27,55).

Kata Kunci : kenari, persilangan, variasi fenotipe.

Artikel

Recording produktivitas breeding domba secara berkelompok

Recording Ternak Domba atau Kambing per induk

Phenotype and Genotype Expression with the GDF9 Gene in the Sapudi and Garut Sheeps

Nikhmatul Hairoh1), Mudawamah Mudawamah*1), Sumartono1)
1) Program Studi Magister Peternakan Universitas Islam Malang, Dinoyo, Kec. Lowokwaru, Kota Malang, Jawa Timur 65144
Corresponding author: mudawamah@unisma.ac.id

Submitted 23 Agustus 2023, Accepted 19 Desember 2023

ABSTRACT

Penelitian ini bertujuan untuk mengetahui ekspresi fenotipe dan genetipe pada domba Sapudi dan Garut, serta perbedaan antara ekspresi gen GDF9 dengan sifat fenotipe pada domba Sapudi dan Garut. Metode penelitian yang digunakan adalah metode deskriptif kuantitatif. Materi yang digunakan adalah 48 ekor domba pejantan yang dipeliharan di UPT Pembibitan ternak dan HMT di Jember dan Peternakan Anak Seribu Pulau Farm Kangploso Malang. Sedangkan untuk pengamatan ekspresi gen GDF9 ekson 1 dengan analisa Quantitative Polymerase Chain Reaction (qPCR) menggunakan primer gen GDF9 dengan sampel sebanyak 8 ekor. Analisa data menggunakan analisa uji–t tidak berpasangan dan dilanjutkan dengan uji BNT. Pengamatan sifat fenotipe meliputi panjang badan (PB), lingkar dada (LD) dan tinggi badan (TB). Sifat genotipe yang diamati adalah ekspresi gen GDF9. Hasil penelitian menunjukkan bahwa karakteristik sifat fenotipe domba Sapudi dan Garut terdapat perbedaan sangat nyata (P<0,01). Berdasarkan penelitian terdapat perbedaan ekspresi sifat fenotipe domba Sapudi dan Garut pada panjang badan (Sapudi 63,21a±3,39; Garut77,71b±5,66) cm, lingkar dada domba (Sapudi 76,15a±3,81; Garut 83,00b±8,75) cm dan tinggi badan (Sapudi 64,96a±3,97; Garut 73,50b±5,60) cm. Terdapat perbedaan sangat nyata (P<0,01) sifat genotipe dari hasil qPCR gen GDF9 antara bangsa domba yaitu domba Sapudi 31,66±5,61 dan Garut 23,70±2,40.

Kata Kunci : GDF9; ukuran tubuh; Domba

ABSTRACT

This study aims to determine the expression of phenotypes and genotypes in Sapudi and Garut sheep, as well as differences between GDF9 exon 1 gene expression and phenotypic traits in Sapudi and Garut sheep. The research method used is a quantitative descriptive method. The material used was 48 rams kept at UPT Breeding Cattle and Forages in Jember and Seribu Pulau Children’s Farm, Kangploso Farm, Malang. As for the observation of GDF9 gene expression by qPCR analysis using GDF9 gene primers with a sample of 8 tails. Data analysis used unpaired t-test analysis and continued with the BNT test. Observation of phenotypic traits included body length (PB), chest circumference (LD), and body height (TB). The genotype observed was GDF9 gene expression. The results showed very significant differences in the phenotypic characteristics of the Sapudi and Garut sheep (P<0.01). Based on the study, there were differences in the expression of the phenotypic traits of Sapudi and Garut sheep on body length (Sapudi 63.21a ± 3.39; Garut 77.71b ± 5.66) cm, chest circumference (Sapudi 76.15a ±  3.81; Garut 83, 00b ± 8.75) cm and height (Sapudi 64.96a ± 3.97; Garut 73.50b ± 5.60) cm. There was a very significant difference (P<0.01) in genotypic traits from the qPCR results of the GDF9 gene between sheep breeds, namely Sapudi sheep 31.66 ± 5.61 and Garut sheep 23.70 ± 2.40.

Keywords: GDF9; body size; beef lamb; qPCR.

article

Arnes Anggita1, Mudawamah2*, & Sumartono3
1,2,&3Program Studi Magister Peternakan, Fakultas Pascasarjana, Universitas Islam
Malang, Jalan Mayjen Haryono Nomor 193, Malang, Jawa Timur 65144,
Indonesia
*Email: mudawamah@unisma.ac.id

Submit: 19–09–2023; Revised: 13–10–2023; Accepted: 06–11–2023; Published: 30–12–2023

ABSTRAK: Indonesia memiliki beberapa jenis domba lokal, di antaranya domba Sapudi. Dalam peningkatan produksi dan populasi domba, perlu diperhatikan sistem reproduksi. Metabolisme dan fisiologi domba bunting akan mengalami perubahan yang menyebabkan komponen darah jugaberubah. Sampel penelitian, yaitu 20 ekor induk domba Sapudi yang berasal dari UPT. Pembibitan Ternak dan Hijauan Makanan Ternak Jember. Induk domba dibagi menjadi 5 kelompok sesuai fase reproduksinya, yaitu induk tidak bunting, kebuntingan awal, pertengahan bunting, kebuntingan akhir, dan induk laktasi. Variabel penelitian adalah komponen darah yang meliputi eritrosit, leukosit, hemoglobin, dan hematokrit. Metode penelitian, yaitu deskriptif kuantitatif yang
dianalisa menggunakan ragam satu arah, dan jika berbeda nyata dilanjutkan uji Duncan. Hasil penelitian ini terdapat perbedaan signifikan (p<0,05) pada hemoglobin, sedangkan pada eritrosit, leukosit, dan hematokrit tidak terdapat perbedaan signifikan (p>0,05). Rataan nilai hemoglobin pada kelima fase reproduksi, yaitu antara 7,65–10,50 g/dL, eritrosit 5,78–6,95 106/mm3, leukosit 8,05–13,71 106/μL, dan hematokrit 24,35–29,70%.

Kata Kunci: Eritrosit, Leukosit, Hemoglobin, Hematokrit, Domba Sapudi.

ABSTRACT: Indonesia has several types of local sheep, including Sapudi sheep. In increasing sheep production and population, it is necessary to pay attention to the reproductive system. The metabolism and physiology of pregnant sheep will experience changes which cause blood components to also change. The research sample was 20 Sapudi ewes from UPT. Livestock Breeding and Forage in Jember. Mother sheep were divided into 5 groups according to their reproductive phase, namely non–pregnant mothers, early pregnancy, mid–pregnancy, late pregnancy, and lactating mothers. The research variables are blood components which include erythrocytes, leukocytes, hemoglobin and hematocrit. The research method is quantitative descriptive which is analyzed using one–way variance, and if it is significantly different, the Duncan test is continued. The results of this study showed significant differences (p<0.05) in hemoglobin, while there were no significant differences in erythrocytes, leukocytes and hematocrit (p>0.05). The average hemoglobin value in the five reproductive phases is between 7.65–10.50 g/dL, erythrocytes 5.78–6.95 106/mm3, leukocytes 8.05–13.71 106/μL, and hematocrit 24.35 –29.70%.

Keywords: Erythrocytes, Leukocytes, Haemoglobin, Hematocrit, Sapudi Sheep.

How to Cite: Anggita, A., Mudawamah., & Sumartono. (2023). Analisis Komponen Darah dari Berbagai Fase Reproduksi Induk Domba (Ovis aries) Sapudi. Bioscientist : Jurnal Ilmiah Biologi,
11(2), 1311–1319. https://doi.org/10.33394/bioscientist.v11i2.9107

article

Mudawamah 1*, Didik Roihuddin2, Nurul Humaidah2, Zulchaidi2, Sumartono2, and GatotCiptadi3

1Postgraduate of Animal Husbandry, University of Islam Malang, Indonesia 2Faculty of Animal Husbandry, University of Islam Malang, Indonesia 3Faculty of Animal Husbandry, Brawijaya University Malang, Indonesia

*Corresponding email: mudawamah@unisma.ac.id

Abstract

The purpose of this study was to describe the phenotypic profile of body weight at one year of male and female came from fraternal twins in Indonesian Local Ettawah Goat (ILEG) or PE Goat. This research method was a case study with data retrieval using purposive sampling with the criteria of male and female fraternal twins. The variables observed were the average and variance of body weight in fraternal twin goats at one year. Data analysed descriptive and unpaired t-test with excel program. The results showed that male goats’ body weight at one year of age was significantly (P < 0.01) higher than that of female goats at fraternal twins. At one year of age, the body weight variance in male goats was higher than that of female goats at fraternal twins. The conclusion was that the phenotype profile of male ILEG goats was more varied, seen from the average and diversity of body weight, which was 15.37% and 52.24% higher than females. This research implies that feeding male goats should be based on body weight, not on age, to increase their potential optimally.
Keyword: PE Goat, twin, variance, body weight

Mudawamah, M. Z. Anwar, and Sumartono

Fakultas Peternakan Universitas Islam Malang

Corresponding Author : mudawamah@unisma.ac.id

ABSTRACT

This research aimed to estimate the repeatability and MPPA values of Sapudi ewes based on the lamb’s daily gain from birth to pre-weaning and weaning. Using recorded data in a pedigree of lamb, 53 data with half-sib relationship came from 14 ewes which included the record of body weight gain of Sapudi lambs from birth to pre-weaning and weaning. The data were analyzed by descriptive method with a quantitative approach. The study showed that the average daily gain of pre-weaning and weaning was 007 kg/day. The repeatability value of pre-weaning and weaning daily gain was 0.636 in the high category and 0.377 in the medium category. The MPPA daily gain values for pre-weaning and weaning ranged from -0.032 to 0.018 and -0.016 to 0.009. The research concluded that the Sapudi sheep ewes based on the MPPA daily gain pre-weaning were six heads with four rankings, while the daily weight gain weaning was ten heads with ranking of five. The highest rank of Sapudi ewes in pre-weaning and weaning daily gain was ear tag 094 with MPPA values of 0.018 and 0.009. It is recommended that further research can be conducted to estimate the repeatability value of quantitative periodically as the basis for the selection and culling of livestock.

Keywords: Ewe, local sheep, repeatability, selection.

Puji syukur Alhamdulillah penulis panjatkan kehadirat Allah SWT atas karuniaNya sehingga diklat petunjuk praktikum ini dapat terselesaikan.
Buku petunjuk praktikum ini disusun dalam rangka memberi penuntun kerja bagi mahasiswa yang menjalankan praktikum genetika dan cara kerjanya berdasarkan kelengkapan peralatan yang ada di Laboratorium Terapan Fakultas Peternakan Universitas Islam Malang dan Preparat yang umum dihadapi di bidang Peternakan.
Tiada gading yang tak retak, semoga buku petunjuk praktikum ini bermanfaat bagi Mahasiswa Fakultas Peternakan Universitas Islam Malang