Mudawamah 1 , I.D. Retnaningtyas 1 , V.M.A. Nurgiartiningsih 2 and C.D.K. Bottema 3
1 Faculty of Animal Science, Islamic University of Malang, Indonesia, 2 Faculty of Animal Science, Brawijaya University of Malang, Indonesia, 3 Faculty of Veterinary, University of Adelaide, Australia
Corresponding email: mudawamah@gmail.com

ABSTRACT

Goat genetic resources in East Java Indonesia are diverse based on genotype and phenotype. It is probably due to crossing among existing breeds existing in this province. This situation cause a problem in the identification of the goat breeds. The objective of this study was to characterize phenotype of PE goat and to analyze their similarity within populations in the different locations. Data of 472 does were analyzed using NTSYS pc version 2.0 software. Dendogram illustrated phenotypic similarity for qualitative traits within fourteen populations in the different of goat breeding areas. The observed qualitative traits were shape of the back, head, face, forehead, horns, ears, and teats. Beside that this study also observed teat and extra teat number, hair color of face, body, legs and tail. The presence and absence of qualitative traits was recorded as “1” and “0”, respectively. The binary coded characters (1, 0) were used for phenotypic similarity analysis. The results indicated that phenotypic similarity among
individuals of PE goat populations were varied and ranged between 0.50-0.84 with an
average of 0.68 + 0.11. It was concluded that phenotypic similarity of PE goats in different breeding locations of East Java was categorized as medium.

Key Words: Goat, Phenotypic similarity, Qualitative, Dendogram

G. Ciptadi 1 , M. Nur Ihsan 1 , A. Nurgiartiningsih 1 and Mudawamah 2
1 Faculty of Animal Husbandry Brawijaya University, 2 Faculty of Animal Husbandry, Unisma, Malang, Indonesia
Corresponding email: ciptadi@ub.ac.id

ABSTRACT

Chromosomal analysis of breeding bull should be performed, because of the importantce of chromosome abnormalities and their negative effect on production and reproduction performance. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. Chromosomal abberation of candidate bull can be identified and culled from breeding program. It is neccessary that bull for straw production for Artificial Insemination (AI) purpose must be screened out for any possible chromosomal abnormality. The cytogenetic control is an important selective measure choice of genetically health breeding bulls.The chromosome analysis to be important to execute. Method performed by collecting blood samples from 3 local cattle breedsand 3 swamp buffalo with diferent phenotypic character of skin colors. Sample of 0.5 ml of blood/animal was added to 5 ml chromosomal medium (Karyo Max, Gibco), placed in incubator at 38 o C. After 70 hours, culture tube were added to 1 ml colchicines kept for 2-3 hours, then were centrifuge at 1000 RPM for 10 minute and supernatant was discarded, pellet cells added then by fixative solution. Slides were prepared by stained with Giemsa for 10 minute, were examined under high power phase-contrast microscope. Chromosomal analysis was performed with help of cytovision software image analysis.Results showed that the 2 N diploid number of chromosome of all three local cattle breed was considered normal was 60, there were 58 autosome and 2 sex chromosome in all breeds cattle observed. Meanhile, buffalo observed for diploid number chromosome 2N= 48, considered as normal swamp buffalo. It was observed that both cattle and buffalo tested were normal categories. The karyotype of all animals showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. However, it was recommended to performed chromosomal investigation of breeding bulls and others Indonesian local breed using advanced sophisticated tools of image analysis technique especially for semen production and AI application.

Key Words: Local cattle, Buffalo, Karyotiping, Chromosome, Abnormalities

Mudawamah 1 , I.Dinasari Ratnaningtyas 1 . M. Zainul Fadli 2 , Aulanni’am 3 and G. Ciptadi 4
1 Faculty of Animal Husbandry, University of Islam Malang, Indonesia
2 Faculty of Medicine, Islamic University of Malang, Indonesia
2 Faculty of MIPA-Chemistry, Brawijaya University, Indonesia
3 Faculty of Animal Husbandry, Brawijaya University of Malang, Indonesia
1 Corresponding author : mudawamah@gmail.com

Abstract
Goat breeding systems in Indonesia have two models with farmer-owned bucks or
government selected bucks in the form of frozen semen. An interesting problem was how the genetic variation of the different goat populations. The purpose of this study was to determine the profile of genetic variation through DNA fragments that appear in gel electrophoresis. Blood samples from each group collected through field studies, followed by analysis of DNA with RAPD. The Primers used in this analysis contained various series primer OPP (OPP series not more 10 and OPP series more 10), and the amplification primers were a recommendation. Each fragment appears from the results of amplification were given a score of 1 and a score of 0 for no fragment appear. Data were analyzed by unpaired t-test using SPSS software V.23. The results showed that the fragments varied from 200 bp to 3000 bp. The DNA fragments used primer OPP which series numbers not more than 10 which significant differences (P <0.05) between two different the goat breeding. There was no difference (P> 0.05) the number of fragments that emerged from the RAPD with OPP which the serial number less than 10 and a combined OPP. The conclusion of this study was the genetic variation of PE population with farmer-owned buck breeding was higher than the government selected buck breeding under RAPD analysis used OPP primer series not more than 10.

Keywords: Local Ettawah Goat, RAPD, OPP

Kuliah Kerja Nyata di Desa Bambang Kecamatan Wajak Kabupaten Malang diiukuti oleh 28 mahasiswa, foto pada saat acara kedatangan disambut oleh Kepala Desa Bambang Bapak Sugianto dan Pak Modin, tengah dosen DPL Universitas islam Malang, Dr. Ir. Mudawamah, M.Si. Kegiatan Kuliah Kerja Nyata dengan tema “Identifikasi Potensi Desa Guna Mewujudkan Desa Mandiri” yang akan dilaksanakan pada tanggal 8 Agustus – 8 September 2018