Mohammad Zainul Fadli1, Mudawamah Mudawamah2*, Irawati Dinasari Retnaningtyas2 , Gatot Ciptadi3 and Oktavia Rahayu Puspitarini2
1 Depart. of Animal Husbandry, University of Islam Malang
2 Depart. of Medical, University of Islam Malang
3 Depart. of Animal Husbandry, Brawijaya University
Correspondent author: Mudawamah@unisma.ac.id

ABSTRACT
In Indonesia, rabbits have developed as small-scale businesses in tourist areas in the form of ornamental rabbits or meat rabbits. The rabbits are kept various kinds of purebred or crossbreeding between purebred and local Indonesian rabbits. The purpose of this study was to observe the genotype F1 crossbreed through the molecular analysis of various rabbit breeds that had been carried out by rabbit breeders as genotype diversity. This research method was a case study and experiment using various rabbits that many farmers raised. The purebred of rabbits used include Rex, Satin, Lion, Flemish Giant, New Zealand White, local Indonesian rabbits. The rabbit crossed include Rex with Satin, Lion with Indonesian Local-bred, Flemish Giant with Local. The Phenotype analysis for F1 derived from purebred and Local-bred under the same management. Molecular analysis was carried out using Polymerase Chain Reaction (PCR) and Polymerase Chain Reaction-Restricted Fragment Length Polymorphism (PCR-RLPF) with GH
primers with HaeIII restriction enzymes. Descriptive analyzed was used for describing research data. The results showed that all samples carried out PCR with GH5 were amplified at 289 bp. PCR results showed that almost all local rabbits were AB-type (85% of the total sample) while all rabbits were purebred and F1 crossbreed with Local-bred had BB type, and phenotype superiority F1 to the dam was 18.51-7.41 %. The conclusion from this study was the F1 derived from purebreds, and Local-bred had a superiority genotype and phenotype compared to the dams (Local-bred).

Keywords: Rabbit, GH, HaeIII, PCR-RFLP, crossbreeding