Analisis Kemiripan Genetika Antara Kambing Peranakan Ettawa Hasil Kawin Alam Dengan Inseminasi Buatan Berdasarkan RAPD

ANALISIS KEMIRIPAN GENETIKA ANTARA KAMBING PERANAKAN ETTAWA HASIL KAWIN ALAM DENGAN INSEMINASI BUATAN BERDASARKAN RAPD

Analysis of Genetic Similarity between PE Goats Derived from Natural Service and Artificial Insemination by RAPD-DNA

Mudawamah 1 , I.D. Retnaningtyas 2 , M.F. Wadjdi 2 , Badriyah 2 , S. Susilowati 2 , Aulanni’am 3 , dan Gatot Ciptadi 4

1 Laboratorium Molekuler Halal Center, Universitas Islam Malang, Malang
2 Fakultas Peternakan Universitas Islam Malang
3 Laboratorium Biokimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya, Malang
4 Laboratorium Pemuliaan Ternak Fakultas Peternakan Universitas Brawijaya, Malang
E-mail: mudawamah@gmail.com

ABSTRAK
Analisis genetika menggunakan random amplified polymorphic DNA (RAPD) untuk mengetahui kemiripan genetika antara kambing peranakan Ettawa (PE) hasil inseminasi buatan (IB) dan kawin alam (KA). Hasil penelitian menunjukkan bahwa dari 20 primer OPA hanya 10 primer yang bisa digunakan untuk mengamplifikasi fragmen deoxyribonucleic acid (DNA) dalam kelompok kambing PE, yang terdiri atas 21 kambing hasil KA dan 19 kambing hasil IB. Berdasarkan profil DNA yang diperoleh dan hasil analisis dengan software numerical taxonomy and multivariate analysis system (NTSYS) disimpulkan bahwa hubungan genetika antara kambing PE hasil kawin alam dengan inseminasi buatan
adalah sebesar 48% dan dikategorikan rendah.
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Kata kunci: kambing, PE, RAPD, kemiripan genetika

Analysis of Genetic Similarity between PE Goats Derived from Natural Service and Artificial Insemination by RAPD-DNA

 

 

 

 

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