Kelinci Kenari

Ternak Lokal Indonesia

Kelinci Kenari

The Different Calcium+2 Intensity Profile and Quality of Oocyte and Goat Sperms after Cryopreservation

G. Ciptadi1S. Rahayu2Fatchiyah2S. Wahyuningsih1A. Budiarto1M. Nasich1A.R.I. Putri2M. Mudawamah3 and M.N. Ihsan1

Abstract

This research aims were to study the effect of the oocyte and sperms cryopreservation of Indonesian local goat on the post-thawing quality and profile or characters of Calcium+2intensity in relating with their fertility capacity. A study was conducted to test the freezing method and post-thawing viability both stock cells stored in the deep freezer and liquid nitrogen (-80°C of vs -196°C). A fertility test of sperms has been conducted through in vitro of sperm quality, while the oocytes cryopreserved test was done by in vitro maturation (IVM) rate (%). The profile of Calcium 2+ was performed and analysis by Confocal Laser Scanned Microscope (CLSM). The result showed that IVM rate of goat oocyte is considered lower when cryopreserved in –80°C than in –196°C. Meanwhile, sperm is considered having a good quality in 2 methods of cryopreservation with post-thawing motility > 40 % (SNI 2014). There is an important difference between Calcium intensity of fresh and post-thawing both for oocyte and spermatozoa. Calcium +2 profiles is varied individually on the peak of intensity, but it considered expressed the same profile of each fresh and post-thawing cell. In vitro fertilization test need to be performed to complete the viability and fertility competence of these sperm and oocyte freezing stocks.

article

5 thoughts on “The Different Calcium+2 Intensity Profile and Quality of Oocyte and Goat Sperms after Cryopreservation

  1. Reading your article helped me a lot and I agree with you. But I still have some doubts, can you clarify for me? I’ll keep an eye out for your answers.

  2. I am currently writing a paper and a bug appeared in the paper. I found what I wanted from your article. Thank you very much. Your article gave me a lot of inspiration. But hope you can explain your point in more detail because I have some questions, thank you. 20bet

Leave a Reply

Your email address will not be published. Required fields are marked *