Repeated G-nucleotides from DNA Sequences from RAPD Results in Indonesian Local Etawah Goats Derived from Natural Service and Artificial Insemination

Mudawamah Mudawamah 1, a) , Muhammad Zainul Fadli 2, , Gatot Ciptadi 3 , Usman
Ali 1 and Gusfarisa Rafika Putri 1
1 Department of Animal Husbandry, Faculty of Animal Husbandry, Islamic University of Malang, Malang, East Java, Indonesia
2 Department of Medical, Faculty of Medical, Islamic University of Malang, Malang, East Java, Indonesia
3 Deapartment of Animal production, Faculty of Animal Husbandry, Brawijaya University, Malang, East Java, Indonesia
a) Corresponding author:

Abstract. Indonesian local Etawah goats (ILEG) are a livestock that has adapted to the environment and management of community farms in Indonesia, with systems of natural service (NS) and artificial insemination (AI). The livestock potential needs to be studied molecularly as a source of information on genetic diversity. The purpose of this study was to determine the repeated of guanine nucleotides (GG, GGG, GGGG, GGGGG and GGGGGG) from DNA sequenced with RAPD results in two ILEG populations of NS and AI. The method used was an experiment with sequencing analysis based on RAPD results with the marker OPA-19 with five replications. The sequencing results were descriptive analysis using SPSS V. 23 with unpaired t-test on the two ILEG populations. The results showed that the ILEG from NS had a very significantly higher number of repeated G-nucleotides (P < 0.01) than ILEG from AI. While the data of repeated
GGG, GGGG, GGGGG and GGGGGG were not used for unpaired t-tested because it did not pass normality test. Descriptively repeated GGG, GGGG, GGGGG and GGGGG on ILEG from NS results were higher than AI. Particularly in repeated G-nucleotides (GGGG, GGGGG and GGGGGG), in ILEG from NS results were still found to be 100% in all sequencing samples, but in ILEG from AI results showed decreased percentages of 60%, 40% and 0%. The conclusion of this study was that repeated G-nucleotides in ILEG from NS are more common than in ILEG from AI.

Keywords: ILEG, RAPD, sequencing

Individual mutations in Indonesian local ettawah goats based on the GDF9 gene

M Mudawamah 1* , I D Ratnaningtyas 1 , M Z Fadli 2 and G Ciptadi 3
1 Animal Science Department, University of Islam Malang, MT Haryono 193 Malang,
2 Medicine Department, University of Islam Malang, MT Haryono 193 Malang, Indonesia
3 Animal Science Faculty, University of Brawijaya, Veteran Malang, Indonesia
*Corresponding author:

Abstract. A prolific trait was essential to determine the litter size in goats including Indonesian Local Ettawah Goats (ILEG), and one of the genes that influence the prolific trait was the GDF9 gene. The purpose of this study was to describe the amino acid expression of the GDF9 gene sequencing in ILEG compared with Genbank accession number GU784823.2. Capra hircus GDF9. This research method was an experiment which includes the sampling of 21 does who had given birth more than once with low, medium and high prolific categories. Furthermore, samples were PCR with exon 1 GDF9 gene, and sequencing results were carried out by amino acid alignment with BioEdit software. The results showed that there were 3 variants in the exon 1 GDF9 gene in ILEG which was located at the location of 27, 61 and 85 amino acid residues. At the 27th residue there was a change in the proline amino acid to alanine (CCT to GCT), at the 61st residue there was no change in the amino acid Leucine, but there was a change in the base N compound (CTA to CTC), and at 85 residues there was a change in acid residue amino Alanine becomes Glycine (GCT to GGT).